| Characterization of Fusarium oxysporum f. sp. fragariae Based on VegetativeCompatibility Group, Random Amplified Polymorphic DNA and Pathogenicity |
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Gopal Nagarajan, Sung Woo Kang, Myeong Hyeon Nam, Jeong Young Song, Sung Joon Yoo, Hong Gi Kim |
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| Abstract |
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Twenty-two isolates of Fusarium oxysporum f. sp. fragariae were obtained from diseased strawberry plants and their characteristics were investigated by vegetative compatibility group (VCG), random amplified polymorphic DNA (RAPD), and pathogenicity. Three major VCGs (A, B, and C) and one incompatible group were identified by nitrate reductase complementation test. The virulence pattern of the 22 isolates was studied in relation to four cultivars including Dochiodome, Redpearl, Maehyang and Akihime. RAPD markers were used to determine genetic relationship, and created three major clusters among the 22 isolates of F. oxysporum f. sp. fragariae. Isolates belong to VCG-C were strongly pathogenic, and relatively high correlation was existed among VCG and RAPD, and virulence. In addition, VCG and RAPD pattern between pathogenic and non-pathogenic isolates were distinctly different. |
| Key Words:
Fusarium oxysporum f. sp. fragariae, genetic variation, nit mutant, RAPD, VCG |
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