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Detection and Quantification of Apple Stem Grooving Virus in Micropropagated Apple Plantlets Using Reverse-Transcription Droplet Digital PCR |
Sung-Woong Kim, Hyo-Jeong Lee, Kang Hee Cho, Rae-Dong Jeong |
Plant Pathol J. 2022;38(4):417-422. Published online August 1, 2022 DOI: https://doi.org/10.5423/PPJ.NT.05.2022.0072 |
Detection and Quantification of Apple Stem Grooving Virus in Micropropagated Apple Plantlets Using Reverse-Transcription Droplet Digital PCR The application of RT-PCR assay for the detection of Apple stem pitting virus and Apple stem grooving virus in four apple cultivars Development and application of reverse transcription droplet digital PCR assay for sensitive detection of apple scar skin viroid during in vitro propagation of apple plantlets The occurrence of Apple stem pitting virus and Apple stem grooving virus within field-grown apple cultivars evaluated by RT-PCR Evaluation of Reference Genes for the Relative Quantification of Apple stem grooving virus and Apple mosaic virus in Apple Trees Simultaneous detection of six citrus viroids and Apple stem grooving virus from citrus plants by multiplex reverse transcription polymerase chain reaction Rapid and Specific Detection of Apple stem grooving virus by Reverse Transcription-recombinase Polymerase Amplification A simple and reliable protocol for the detection of apple stem grooving virus by RT–PCR and in a multiplex PCR assay Droplet-vitrification cryotherapy for eradication of apple stem grooving virus and apple stem pitting virus from “Marubakaido” apple rootstock First Detection and Molecular Characterization of Apple Stem Grooving Virus, Apple Chlorotic Leaf Spot Virus, and Apple Hammerhead Viroid in Loquat in Spain |