| Phylogenetic and Taxonomic Status of the Phytoplasmas Associated with Water Dropwort(Oenanthe javanica DC) Disease in Korea and Japan |
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Hee Young Jung, Tae Ha Woo, Tadaaki Hibi, Shigetou Namba, Joon Tak Lee |
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| Abstract |
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To evaluate the phylogenetic and taxonomic status of the phytoplasmas associated with water dropwort (Oenanthe javanica DC) disease in Korea and Japan, their 16S rDNA was analyzed. DNAs extracted from water dropworts collected in Korea (Kyongnam province) and Japan (Chiba prefecture) affected by witches` broom and yellows were subjected to PCR using phytoplasma-specific primers, which amplified a 1.4-kbp fragment that included the 16S rDNA. Phytoplasmas were characterized by RFLP analysis using AluI, HaeIII, HhaI, KpnI, MseI, and RsaI restriction enzymes and by sequence analysis of the PCR products. The water dropwort witches` broom (WDWB) and water dropwort yellows (WDY) 16S rDNA sequences were identical and closely related to onion yellows (OY, 99.9% identity), which belong to the aster yellows (AY) 16S-subgroup. However, the KpnI RFLP analyses clearly distinguished the WDY and WDWB phytoplasmas from the OY phytoplasma. The phylogenetic analysis based on 16S rDNA showed that WDWB and WDY phytoplasmas are members of a relatively homogeneous group that evolved from a common ancestor. |
| Key Words:
phylogeny, phytoplasma, water dropwort witches` broom |
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