The Plant Pathology Journal 2004;20(2):155-157.
Published online June 30, 2004.
dsRNA Analysis and Sequence of S12 to Rice dwarf virus Korean Isolate
Bong Choon Lee, Do Yeon Kwak, Yeon Kyu Hong, Hyun Je Cho, Sung Tae Park, Soon Chul Kim
Abstract
We isolated Rice dwarf virus (RDV) from infected plants in rice fields (Korea, Japan, China, the Philippines and Nepal) and analyzed their genomic dsRNAs by polyacrylamide gel eletrophoresis. The genomic dsRNAs of the isolates showed distinct electrophoretic mobility profiles. The S12 coding to nonstructural protein of Korean isolate (RDV-Kr) was further analyzed by sequencing. The S12 of RDV-Kr was 1,066bp long and coded for a protein composed of 312 amino acids including three open reading frames of P12, P12OPa and P12OPb. The sequence identities were 96% and 98.6% with Japanese isolates (H, AN), 94.7% with Nepalese isolate (NEL), 94% with Chinese isolate (CK) and the Philippines isolate (P).
Key Words: dsRNA, RDV-Kor, Rice dwarf virus, S12 sequence
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