Plant Pathol J > Volume 20(3); 2004 > Article
The Plant Pathology Journal 2004;20(3):165-171.
DOI:    Published online September 30, 2004.
Potential Biotypes in Korean Isolates of Bipolaris cactivora Associated with Stem Rot of Cactus
Jeong Ho Kim, Myoung Il Jeoung, Ick Hwa Hyun, Young Ho Kim
A total of 62 isolates of Bipolaris cactivora causing cactus stem rots were isolated from major cactusgrowing areas in Korea. Colony morphology of the isolates on potato-dextrose agar was differentiated into aerial (CA) and non-aerial mycelial types (CB). CA had profound aerial mycelium with grayish brown (CA-1), light brownish (CA-2), and brownish (CA-3) pigmentations; respectively, while CB had dark brownish pigmentations. CA had conidia of less dark pigmentation and acute terminal end. CB had darker and more round-end conidia. Twenty-eight amplified fragments were produced by polymerase chain reaction (PCR) with a set of 2 random primers. The sizes of amplified DNA fragments ranged approximately from 0.1 to 2.3 kbp. The isolates were classified into 2 major genomic DNA random amplified polymorphic DNA (RAPD) groups at the genomic similarity of 97.7% and 95.1%, respectively. Cluster analysis of genetic similarity among the isolates generated a dendrogram that clearly separated all isolates into SA or SB. This result suggests that there may be two morphotypes of B. cactivora in Korea that may differ in their genetic constitutes.
Key Words: Bipolaris cactivora, cactus stem diseases, colony morphology, polymerase chain reaction, PCR, random amplified polymorphic DNA, RAPD
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