The Plant Pathology Journal 2004;20(4):264-270.
Published online December 31, 2004.
Genetic Variation in Fusarium oxysporum f. sp. fragariae Populations Based on RAPD and rDNA RFLP Analyses
Gopal Nagarajan, Myeong Hyeon Nam, Jeong Young Song, Sung Joon Yoo, Hong Gi Kim
Abstract
Fusarium oxysporum f. sp. fragariae is a fungal pathogen causing strawberry wilt disease. The random amplified polymorphic DNA (RAPD) and restriction fragment length polymorphisms (RFLPs) of intergenic spacer (IGS) region of rDNA were used to identify genetic variation among 22 F. oxysporum f. sp. fragariae isolates. All isolates could be distinguished from each other by RAPD analysis and RFLP of 2.6 kb amplified with primer CNS1 and CNL12 for IGS region of rDNA. Cluster analysis using UPGMA showed eight distinct clusters based on the banding patterns obtained from RAPD and rDNA RFLP. These results indicate that F. oxysporum f. sp. fragariae isolates are genetically distinct from each other. There was a high level genetic variation among F. oxysporum f. sp. fragariae.
Key Words: Fusarium oxysporum f. sp. fragariae, genetic variation, RAPD, rDNA, RFLP


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