The Plant Pathology Journal 2004;20(4):308-312.
Published online December 31, 2004.
The Application of RNA Transcript Conformation Polymorphism in ResolvingMixed Infection of PVY Isolates
Arie Rosner, Ludmila Maslenin
Abstract
A method based on RNA-transcript conformation polymorphism (TCP) was tested for detection of two PVY isolates in a mixed infection. Differences in electrophoretic mobility of RNA transcripts copied from PCR products of each virus isolate enabled the distinction between the two virus isolates in a mixed infection. The identities of the RNA transcripts and hence of the infecting virus isolates were determined by annealing to reference oligonucleotides containing unique strain-specific sequences visualized by retardation of transcript mobility in gel. The ratio at which both virus isolates could be detected was as low as 1:10. The suitability of this procedure for the study of mixed virus infections is discussed.
Key Words: Potato virus Y, rSSCP, strain differentiation, transcript conformation polymorphism
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