Relatedness Among Indiginous Members of Sclerotinia sclerotiorum by Mycelial Compatibility and RAPD Analysis in the Jordan Valley

Osofee, H.; Hameed, K. M.; Mahasneh,  A.

doi:2005.21.2.106

Plant Pathol J > Volume 21(2); 2005 > Article

The Plant Pathology Journal 2005;21(2):106-110.
DOI: https://doi.org/10.5423/PPJ.2005.21.2.106 Published online June 30, 2005.

Relatedness Among Indiginous Members of Sclerotinia sclerotiorum by Mycelial Compatibility and RAPD Analysis in the Jordan Valley

H. Osofee, K. M. Hameed, A. Mahasneh

Abstract

Sclerotinia sclerotiorum attacks most of the vegetable crops in the Jordan valley. Twenty-five samples/isolates were obtained in a complete coverage of that region. They were characterized for their mycelium incompatibility, and specific gene amplified using the primer SSREV/SSFWD. All isolates gave similar single band around 278 bp. Thirteen isolates were completely incompatible with the other 12 ones. The latter ones fell into four subgroups of mycelium incompatibility.RAPD analysis using three primers (OPA-2, OPA-10,and OPA-18) clustered the 25 isolates into subgroups in agreement with their morphological separation, indicating close correlation between amplified gene(s) and the gene(s) of incompatibility. All highly virulent isolates were among the group of 13, indicating a well established genomic type pathogen in this region.

Key Words: cottony rot, Cucurbita pipo, Jordan valley, mycelial compatibility, RAPD assay, Sclerotinia sclerotiorum