Occurrence and Detection of Rice black-streaked dwarf virus in Korea

Lee, Bong Choon; Hong, Yeon Kyu; Hong, Sung Jun; Park, Sung Tae; Lee, Key Woon

doi:2005.21.2.172

Plant Pathol J > Volume 21(2); 2005 > Article

The Plant Pathology Journal 2005;21(2):172-173.
DOI: https://doi.org/10.5423/PPJ.2005.21.2.172 Published online June 30, 2005.

Occurrence and Detection of Rice black-streaked dwarf virus in Korea

Bong Choon Lee, Yeon Kyu Hong, Sung Jun Hong, Sung Tae Park, Key Woon Lee

Abstract

Until now, occurrence of Rice black-streaked dwarf virus (RBSDV) is observed in Gyeongsang provinces, southeastern part of Korea. However, recently, the occurrence of RBSDV is increasing and spreading in Jeonra provinces including Gochang-gun, southwestern part of Korea. RBSDV infected plants showed typical symptoms including stunted, deformed leaves with white waxy or black-streaked swelling along the veins. We extracted viral genomic dsRNA from infected leaves and analyzed dsRNA pattern by polyacrylamide gel electrophoresis. Ten genomic segments with similar sized dsRNAs were observed. We also detected RBSDV by reverse transcription (RT)-PCR using specific primers for S10 from genomic dsRNA and observed amplified DNA fragment specific for RBSDV S10.

Key Words: genomic dsRNA, Rice black-streaked dwarf virus, S10