Molecular Characterization and Infectious cDNA Clone of a Korean Isolate of Pepper mild mottle virus from Pepper

Yoon, Ju Yeon; Hong, Jin Sung; Kim, Min Jea; Ha, Ju Hee; Choi, Gug Seon; Choi, Jang Kyung; Ryu, Ki Hyun

doi:2005.21.4.361

Plant Pathol J > Volume 21(4); 2005 > Article

The Plant Pathology Journal 2005;21(4):361-368.
DOI: https://doi.org/10.5423/PPJ.2005.21.4.361 Published online December 31, 2005.

Molecular Characterization and Infectious cDNA Clone of a Korean Isolate of Pepper mild mottle virus from Pepper

Ju Yeon Yoon, Jin Sung Hong, Min Jea Kim, Ju Hee Ha, Gug Seon Choi, Jang Kyung Choi, Ki Hyun Ryu

Abstract

A Korean isolate of Pepper mild mottle virus (PMMoVKr) was isolated from a diseased hot pepper plant and its biological and molecular properties were compared to that of PMMoV-J and PMMoV-S. The genomic RNA of PMMoV-Kr consists of 6,356 nucleotides. The nucleotide and amino acid sequences identities of four viral proteins and two noncoding regions among PMMoV-Kr, PMMoV-S and PMMoV-J were 96.9% to 100.0% and 97.5% to 98.6%, respectively. Full-length cDNA amplicon of PMMoV-Kr was directly amplified by RT-PCR with a set of 5`-end primer anchoring T7 RNA promoter sequence and 3`-end virus-specific primer. Capped transcript RNAs from the full-length cDNA clone were highly infectious and caused characteristic symptoms of wild type PMMoV when mechanically inoculated to systemic host plants such as Nicotiana benthamiana and pepper plants.

Key Words: genome, infectious cDNA clone, pepper, Pepper mild mottle virus, sequence, Tobamovirus