Occurrence of Tomato spotted wilt virus in Chrysanthemum (Dendranthema grandiflorum) in Korea

Chung, Bong Nam; Pak, Ha Seung; Jung, Jae A; Kim, Jeong Soo

doi:2006.22.3.230

Plant Pathol J > Volume 22(3); 2006 > Article

The Plant Pathology Journal 2006;22(3):230-234.
DOI: https://doi.org/10.5423/PPJ.2006.22.3.230 Published online October 1, 2006.

Occurrence of Tomato spotted wilt virus in Chrysanthemum (Dendranthema grandiflorum) in Korea

Bong Nam Chung, Ha Seung Pak, Jae A Jung, Jeong Soo Kim

Abstract

Tomato spotted wilt virus (TSWV) has been identified in commercial chrysanthemum cultivars in Korea. Nucleotide sequences of the N gene of TSWV-ch14 isolated from infected chrysanthemum were determined and deposited in GenBank under accession no. DQ453158. The symptoms consisted of dark colored leaf necrosis, black streaks along the stem, wilting of plant parts in ``Sinma``; and chlorotic spots, necrosis of axillary shoots and withering of leaves in ``Hwarang``. Electron micrographs of leaf preparation of Nicotiana rustica infected with TSWV-ch14 contained spherical particles around 85 nm in diameter. TSWV was identified from chrysanthemum by sequence determination of N nucleocapsid protein and virion observation by transmission electron microscope. This is the first reported observation on TSWV in chrysanthemum in Korea.

Key Words: Chrysanthemum, TSWV