Characterization of Colletotrichum Isolates Causing Anthracnose of Pepper in Korea

Kim, Joon Tae; Park, Sook Young; Choi, Woo Bong; Lee, Yong Hwan; Kim, Heung Tae

doi:2008.24.1.17

Plant Pathol J > Volume 24(1); 2008 > Article

The Plant Pathology Journal 2008;24(1):17-23.
DOI: https://doi.org/10.5423/PPJ.2008.24.1.017 Published online March 31, 2008.

Characterization of Colletotrichum Isolates Causing Anthracnose of Pepper in Korea

Joon Tae Kim, Sook Young Park, Woo Bong Choi, Yong Hwan Lee, Heung Tae Kim

Abstract

re identified based on mycological characteristics, responses to fungicides carbendazim and the mixture of carbendazim and diethofencarb, and nucleotide sequence analysis of internal transcribed spacer (ITS) region. Most of the Colletotrichum isolates from pepper could be identified as C. acutatum. The pepper isolates produced grey white mycelia that gradually changed to dark gray. The conidia were variable in size, and almost cylindrical in shape with at least one rounded end. They could grow on PDA amended with carbendazim or with the mixture of carbendazim and diethofencarb at 10 μg/ ml, to which the isolates from apple and strawberry were very sensitive. A part of the ITS regions from the Colletotrichum isolates was amplified with the specific primers designed for C. acutatum (Ca1-1) or C. gloeosporioides (Cg1-3). A primer pair of Ca1-1 and a universal primer (ITS4) amplified a 496-bp DNA fragment from all of the pepper isolates examined and one apple isolate. Taken together, it is conclusive that the Colletotrichum isolates causing the typical lesion of anthracnose on pepper fruits are C. acutatum.

Key Words: Colletotrichum acutatum, ITS region, pepper anthracnose, PCR with species-specific primer