Plant Pathol J > Volume 31(3); 2015 > Article
Kim, Shim, Kim, Hong, Park, Han, Kim, and Kim: Effect of Aerated Compost Tea on the Growth Promotion of Lettuce, Soybean, and Sweet Corn in Organic Cultivation


This study investigated the chemical characteristics and microbial population during incubation of four kinds of aerated compost teas based on oriental medicinal herbs compost, vermicompost, rice straw compost, and mixtures of three composts (MOVR). It aimed to determine the effects of the aerated compost tea (ACT) based on MOVR on the growth promotion of red leaf lettuce, soybean and sweet corn. Findings showed that the pH level and EC of the compost tea slightly increased based on the incubation time except for rice straw compost tea. All compost teas except for oriental medicinal herbs and rice straw compost tea contained more NO3-N than NH+4-N. Plate counts of bacteria and fungi were significantly higher than the initial compost in ACT. Microbial communities of all ACT were predominantly bacteria. The dominant bacterial genera were analyzed as Bacillus (63.0%), Ochrobactrum (13.0%), Spingomonas (6.0%) and uncultured bacterium (4.0%) by 16S rDNA analysis. The effect of four concentrations, 0.1%, 0.2%, 0.4% and 0.8% MOVR on the growth of red leaf lettuce, soybean and sweet corn was also studied in the greenhouse. The red leaf lettuce with 0.4% MOVR had the most effective concentration on growth parameters in foliage part. However, 0.8% MOVR significantly promoted the growth of root and shoot of both soybean and sweet corn. The soybean treated with higher MOVR concentration was more effective in increasing the root nodule formation by 7.25 times than in the lower MOVR concentrations Results indicated that ACT could be used as liquid nutrient fertilizer with active microorganisms for culture of variable crops under organic farming condition.

Organic cultivation techniques for crops production in the field and greenhouses have been developed in alternative production techniques which employ biological or organic compounds for disease and pest control (Litterick et al., 2004; Termorshuizen et al., 2006; Turemis, 2002).
Lately, current environmental issues are capturing the world’s attention focusing on improving the environmental quality through the adoption of techniques and measures that have reduced impacts on the environment (Hamdar et al., 2000). Nutrient value of organic composts varies widely depending upon the nature of the composted feedstock. If the start material contains manure, most finished composts are richer in nitrogen and other nutrients and have better chemical characteristics (Abu-Zahra and Tahboub, 2008; Steinegger and Janssen, 1996). The role of organic matter is very important. Its high content in the soil influences physical properties (Mc Conenell et al., 1993; Uehara and Gilman, 1981); assures good value of soil cation exchange capacity (CEC); reduces the mobility of nutrients in soil solution (Duxbury et al., 1989), prevents the loss of useful substances by means of the action of enzymes; avoids the pollution of water table; improves soil porosity (Bauer and Black, 1994); helps the chemical stabilization of structure; and reduces the processes of soil erosion and increases the micro-organisms and enzymatic activity (Brown et al., 1993).
Composts may induce systemic resistance in plants (Pharand et al., 2002), and composted yard waste applied to soil prior to planting also reduces bacterial spot incidence on tomato fruit, although foliar disease is not reduced (Abbasi et al., 2002). Compost tea is one of the alternative techniques extracted from composted materials by steeping or brewing the compost in water using various preparation methods (Ingham, 2005; Litterick et al., 2004; Scheuerell and Mahaffee, 2002). Study on compost tea technology began in 1980’s in USA, but field practices comparing the brewing methods are few. Historically, home-made extract of compost tea called “passive” or non-aerated compost tea (NCT) were prepared by suspending a bag of compost in a container of water for 14 days to extract anaerobic microbes and nutrients which are used and applied to promote plant health and vitality in plants (Ingham, 2005; Naidu, 2010; Scheuerell and Mahaffee, 2002). Recently, aerated compost tea has been brewed in large-scale mechanized equipment for shorter period of time and often supplemented with oxygen, nutrients, and microbial starter cultures to enhance the biological activity of the compost tea which contains aerobic microbes and nutrients (Ingham, 2005; Naidu et al., 2010; Scheuerell and Mahaffee, 2004).
Compost tea has been cited as an option for conventional and organic growers thought to enhance crop fertility by introducing microorganisms that might aid in soil nutrient retention and extraction, and by adding soluble nutrients, further adding to their potential value as a part of an integrated crop management plan (Diver, 2002; Ingham, 2005; Kannangara et al., 2006; Merrill and McKeon, 2001).
To date, majority of the studies have focused on plant disease control and suppressive ability of compost tea (Goonani et al., 2011; Litterick et al., 2004; Weltzien, 1992). A little plant disease control studies are available on aerated compost tea or non-aerated compost tea. Conforti et al. (2002) reported that a field practice at Presidio Golf Course, to assess the efficacy of aerated compost tea on turf grass growth and in suppressions of a common fungal disease, Michrodocim patch caused by Michrodocium nivale. Plamer et al. (2010) presented that aerated compost tea introduced from immature compost suppressed the growth of Botrytis cinerea on bean leaflets, with an abundant and diverse microbial community.
There are number of reports that some plant diseases have been partially suppressed by application of compost tea (Brinton et al., 2004; Scheuerell and Mahaffee, 2002). Al-Dahmani et al. (2003) demonstrated that the population density of Xanthomonas vesicatoria in infected leaves was reduced significantly by foliar sprays with compost extracts produced from composted cow manure. Zaller (2006) also reported that the use of foliar vermicompost extracts sprayed tomato plants showed clear signs of Phytophthora infestans infection as water sprayed plants; the severity of the infection was unaffected by the two spraying treatments produced from fruit, vegetable and cotton waste by redworms (Eisenia fetida Sav.).
In other studies, however, suppression has been highly variable between different batches or batch preparations (Scheuerell and Mahaffee, 2004, 2006). Potential problems such as pathogen or chemical contamination of crops by application of compost tea have also been documented in recent scientific studies (Ingram and Millner, 2007; Rahel et al., 2007).
The objectives of this study was to determine the efficacy dose of application and evaluated the efficacy of aerated compost teas prepared from oriental medicinal herbs compost, vermicompost, rice straw compost, and mixtures of three composts (MOVR) on the growth promotion of red leaf lettuce, sweet corn and soybean in the greenhouse.

Materials and Methods

Preparation of plant materials

Red leaf lettuce, beet and sweet corn seeds were obtained from commercial seed company in Korea. Twenty seeds of red leaf lettuce, beet and sweet corn were sown in 72 holes nursery trays using horticultural nursery media based with coconut beat and peat moss. The seedling of each crop was regularly watered as required. Temperature and relative humidity in the greenhouse were 28°C and 80%, respectively under 18/6 h (light/dark) photoperiod.

Preparation of composted materials and compost tea

Three types of organic compost materials, rice straw compost, vermicompost and Hinoki cypress bark compost were prepared using same materials and allowed to mature for two years using windrow composting system. The organic compost mixtures (MOVR) obtained from two-year old composted rice straw compost, vermicompost and Hinoki cypress bark compost were used for the production of aerated compost tea. Aerated compost tea was prepared by mixing one liter of homogenized compost mixture with 10 l of tap water (from agricultural well) in a 20 l handmade compost tea brewer. Actively aerated compost tea was allowed to brew at room temperature for four days of incubation in 20 l of handmade compost tea brewer with air pump. Following the incubation period, actively aerated compost was filtered through two-layer 800 micron mesh bag which reduces the chance of clogging application equipment for foliage spray.

Analysis of chemical properties of aerated compost tea

The pH level and electrical conductivity (EC) of the aerated compost tea were measured in each batch of 1:1 (v/v) mixture of deionized water: aerated compost tea using a conductivity/pH meter (3200 M Multi-Parameter Analyzer, Agilent Technologies, U.S.A.). Mineral N (NH4-N, and NO3-N) of the aerated compost tea was analyzed calorimetrically using a discrete analyzer (Easy Chem Plus, Systea Scientific, IL).

Analysis of microbial population density of compost tea

Microbial population density of each aerated compost tea was analyzed on three samples from three separate events by extraction methods. Compost tea samples were taken after 24 h brewing cycle for each aerated compost tea. One milliliter sample of each aerated compost tea was transferred into 9 μl distilled water. The 100 μl of serial dilution of each compost tea was spread evenly on four plates of nutrient agar (NA, nutrient broth; 8 g, agar; 15 g, distilled water 1 l) (Difco, USA), potato dextrose agar (PDA, potato starch; 4 g, dextrose; 20 g, agar; 15 g, distilled water 1 l) (Difco, USA) and rose bengal agar (RBA) based PDA and added 67 mg/l rose bengal (Smith and Dawson, 1944). After incubation at 25°C for 24 h, population densities were reported as colony forming unit (cfu)/ml from each media.

Identification of bacterial isolates by 16S rDNA sequencing

A single colony of purified each isolate was inoculated into 9 mL LB media and incubated in shaker incubator at 27°C for 5 days with constant shaking (180 rpm). 100 to 1,200 purified isolates were randomly picked, checked for purity, and stored in 25% glycerol at −80°C until further analysis.
Genomic DNA was extracted using enzymatic lysis and alkaline hydrolysis method as modified by Kim et al. (2011). A 1 ml of bacterial cells was lysed in 200 μl of lysis buffer (0.05 M Tris-HCl, 1 mM EDTA, pH 7.5) containing 0.5 mg of lysozyme (Sigma-Aldrich Chemie GmbH, Schnelldorf, Germany) by incubation for 1 h at 37°C. After addition of 10 μl of each of 1 M NaOH and 10% sodium dodecyl sulfate, the mixture was incubated at 95°C for 10 min and neutralized with 10 μl of 1 M HCl. Nucleic acids were then purified using the QIAamp DNA bacteria mini kit (Qiagen, Basel, Switzerland), resulting in a sample volume of 100 μl.
The 16S rRNA gene was amplified using conventional PCR with primers fD1 (5′-AGAGTTTGATCCTGGCTCAG-3′) and rP2 (5′-ACGGCTACCTTGTTACGACTT-3′), producing an amplicon of approximately 1,500 bp (Weisburg et al., 1991). Cycling parameters included an initial denaturation for 5 min at 95°C, 40 cycles of 1 min at 94°C, 1 min at 60°C, and 1 min at 72°C, and a final extension for 10 min at 72°C. Five microliters of the DNA extract was used for amplification in a total volume of 50 μl containing 1.25 U of AmpliTaq DNA polymerase LD (Applied Biosystems, Rotkreuz, Switzerland), and the appropriate buffer. Amplicons were purified with the QIAquick PCR purification kit (Qiagen AG, Basel, Switzerland) and sequenced with forward primer fD1 using the BigDye kit and an automatic ABI Prism 3730 Genetic Analyzer (Applied Biosystems, Rotkreuz, Switzerland).
PCR products were purified and sequenced from both ends using an ABI 3730 automated sequencer (Applied Biosystems Inc., Rotkreuz, Switzerland). The sequences were manually checked for quality, assembled, trimmed, and aligned using BioEdit ( The sequences were subjected to Blast searches against the NCBI database.

Evaluation of plant growth with compost tea

Four treatments at the rate of 50 ml per plant were applied in the early morning every week for four weeks starting three days after transplanting (DAT) each crop seedlings to the root zone and foliage of plants. The compost tea extraction ratio was 1:1 (v/v) aerated compost tea to tap water and 50 mL of aerated compost tea was applied to the root zone and was applied to the foliage. Aerated compost teas were prepared separately events every week before application using the same batch of the mixture of four compost materials in a shade close to the greenhouse. Plants were harvested five weeks after treatment. The quantitative properties of red leaf lettuce, sweet corn, and soybean were determined in each plant by measuring the fresh weight and leaf number, leaf length, leaf width or shoot, and root length of the tested crops.

Experimental design and statistical analysis

Experiments were conducted using a completely randomized design with 15 replicates of each treatment. Pairwise comparisons between treatments were performed using the least significant difference test. When significant differences were observed (p≤0.001), treatment means experiments were separated using Fisher’s least significant differences (LSD) test at 95% confidence level (p≤0.05) using the Statistical Analysis System, version 9.1 (SAS statistical analysis software, SAS Institute Inc. Cary, NC).

Results and Discussion

Chemical properties of aerated compost teas

The changes in pH level and electronic conductivity (EC) of the compost teas are shown in Fig. 1. After a day of incubation, pH levels of all aerated compost teas slightly increased (2.2-9.9%) in the following order, rice straw compost, oriental medicinal herbs compost, vermicompost, and MOVR during incubation period (Fig. 1A). Vermicompost tea and oriental medicinal herbs compost tea had the highest and lowest pH levels pH 8.54 (±0.88) and pH 7.90 (±0.88), respectively for five days after aeration (Fig. 1A). Also, the EC values of all aerated compost teas except for the rice straw compost tea significantly increased (18.3-49.2%) after one day incubation (Fig. 1B). Highest EC level was observed in the mixture of oriental medicinal herbs compost and vermicompost scored 2.22 ms/l (±0.01) for five days after incubation (Fig. 1B). Likewise, the EC values for rice straw compost tea significantly decreased from 0.64 ms/l to 0.33 ms/l after incubation (Fig. 1B).
Koné et al. (2010) reported that pH of non-aerated compost tea ranged from 5.92 (sheep manure) to 6.57 (chicken manure) and the highest EC was found in chicken manure (1.05 ms/l) compost tea followed by seaweed compost tea (0.48 ms/l). Also, Haggag and Saber (2007) described that the EC and pH of compost tea based on plant residues (rice ash, bean straw, and vegetative food waste) was higher than chicken manure. However, Pant et al. (2011) reported that application of vermicompost teas did not affect pH, but significantly increased EC, N and K content of Pak Choi growth media. Hence, physical and chemical properties of nutrients in the compost tea could improve the nutritional status of the plants (Koné et al., 2010).
Fig. 2 shows the changes in soluble nitrogen types, NO3-N and NH+4-N of the compost teas. All compost teas except for oriental medicinal herbs compost tea contained more NO3-N type of nitrogen than NH+4-N type of nitrogen. Among the compost teas, rice straw compost tea had very low NO3-N and NH+4-N nitrogen contents scored 4 mg/l and 1 mg/l, respectively, for 5 days after brewing. Moreover, rice straw and oriental medicinal herbs compost tea had very low NO3-N nitrogen contents compared to the other compost teas. The highest NO3-N nitrogen content was observed in vermicompost tea scored as 609.4 (±4.1) mg/l after 2 days aeration (Fig. 2A). The mixture of oriental medicinal herbs compost and vermicompost highly decreased from 737.7 mg/l to 392.4 mg/l (−46.8%) after one day incubation (Fig. 2A).
The MOVR had the highest NH+4-N nitrogen contents scored 27 (±2.0) mg/l for 5 days after incubation (Fig. 2B). The oriental medicinal herbs, the mixture of oriental medicinal herbs compost, and vermicompost tea showed decreased in NH+4-N nitrogen contents after incubation (Fig. 2B). Little change of NH+4-N nitrogen content was observed for rice straw compost and vermicompost tea after incubation (Fig. 2B).
In a research conducted by Gorlach and Mazur (2002), nitrogen losses were greater in applied fertilizer containing oxidized form of nitrogen (NO3-N) than in those containing reduced form of nitrogen (NH+4-N and NH2+2-N) present in physiologically acid fertilizers such as urea and ammonium sulfate. Owing to these chemical properties, nitrogen fertilizers could affect the soil pH and influence the availability of microelements and heavy metals in plants (Smole’n et al., 2012).

Microbial population density of compost tea

Culturable microbial population densities were significantly different between the compost materials (Fig. 3). Microbial communities of all aerated compost teas were predominantly bacteria (Fig. 3A). The average population densities of culturable bacteria increased in all the aerated compost teas for three days after incubation. Highest population density of culturable bacteria was recorded 1.5 (±13.6)-5.3 (±20.5) × 107 cfu/ml in the mixture of oriental medicinal herbs compost and vermicompost tea during incubation (Fig. 3A) However, fungal population densities were significantly lower than those of bacteria and significantly decreased in all compost teas after two days incubation. The highest population density of fungi was recorded 8.7 (±0.6)-24.7 (±2.1) × 103 cfu/ml in the mixture of oriental medicinal herbs compost and vermicompost tea during incubation (Fig. 3A).
Ingham (2005) reported that aerated compost tea often added sugars, grain, fish emulsion, kelp tea, humic acid and other materials is enhanced microbial activity of the final brewing product. The compost extracts were undoubtedly harbored significant populations of biocontrol agents because they were prepared from mature composts, which typically are colonized by a great diversity of such microorganisms (Alvarez et al., 1995; Boehm et al., 1997; Bulluck and Ristaino, 2002; Hoitink et al., 1997; Hoitink and Boehm, 1999).
In the study, all compost teas exceeded the threshold and showed high microbial loads. Thus, all microbial groups with the exception of fungi have values greater than 106 cfu/mL or even around 107 cfu/ml.
The level of microbial population in compost teas necessary to determine suppressive level has been extensively studied (Diánez et al., 2007; Scheuerell and Mahaffee, 2004). Microbial communities inhabiting the compost colonized water extracts had proliferated during the compost tea incubation. Final aerated compost teas were applied in crops to achieve plant growth promotion and plant pathogen control. Also, Sylvia (2004) reported that compost extract containing high population of microbiota such as Rhizobacteria, Trichoderma, and Pseudomonas spp. enhanced the growth and yield of crops. Scheuerell and Mahaffee (2004) hypothesized that 106 cfu/ml of culturable bacteria represents a transition, counting data from non-suppressive to suppressive compost teas.

The dominant bacterial groups of the MOVR compost tea

In the culture-dependent analysis of microbial diversity, 100 bacterial strains were isolated from MOVR compost tea and were identified as 15 groups including uncultured bacterium at the genus level by 16S rDNA gene-based sequences analysis. The dominant bacterial genera were Bacillus (63.0%), Ochrobactrum (13.0%), Spingomonas (6.0%) and uncultured bacterium (4.0%) in Fig. 4.
Ryckeboer et al. (2003) attempted to determine the microbial community of the dominant bacteria group and functional groups of microorganisms originated from composting of bio-waste, using incubation, isolation, and enumeration techniques. They reported that dominant bacteria groups were fungi, Streptomyces, and yeasts during the themorphilic phase of composting procedures. Bacillus genera was dominated during the peak-heating phase of fresh wastes.
Cahyani et al. (2003) reported that a successional trabsition of bacterial community groups: Alphaproteobacteria in the raw materials of rice straw compost, Bacillus and Actinomycetes at the thermophilic phase, and Cytophage and Clostridial groups at the middle and curing phase during composting of rice straw.

Growth promotion effect of compost tea on plant

Among the four compost teas, the mixture of oriental medicinal herbs compost and vermicompost tea (MOVR) had significantly higher levels of nitrogen content and microbial population density. Thus, MOVR was selected as the candidate compost tea for growth promotion test of plants in the study. Applications of the MOVR significantly increased shoot and root growths of the tested plant such as red leaf lettuce (Table 1), sweet corn (Table 2) and soybean (Table 3, Fig. 3) compared to the untreated control. The fresh weight of the upper part of red leaf lettuce foliage was significantly greater at 0.4% MOVR than the other MOVR concentration (Table 1). The effect of four MOVR concentrations (0.1%, 0.2%, 0.4%, and 0.8%) on shoot growth of the red leaf lettuce resulted in greater leaf number, length and width than the untreated control (Table 1). Application of 0.8% MOVR concentration significantly increased the growth of root and shoot of both sweet corn (Table 2) and soybean (Table 3). The soybean treated with higher MOVR concentration was more effective in increasing the root nodule formation by 7.25 times than in the lower MOVR concentrations (Table 3 and Fig. 5).
Similar results were demonstrated that vermicompost tea found to increase plant growth best under laboratory tests was applied to cereals (wheat and barley) and vegetables (Raphanus sativus, Rucola selvatica, and Pisum sativum) in a field study (Fritz et al., 2012).
Efficacy of compost extracts may vary considerably. This may be due to differences in procedures used for preparation of the compost extracts, the source, nutrient composition, quality, and maturity of the compost (Weltzien, 1992) According to research compiled at the Kentucky State University, nutrients in compost tea have been detected in plant roots as early as one hour immediately after application (Micheal, 2001). The liquid fertilizer directly applied to the plant’s foliage quickly provided the cell tissues with nutrients. This reaction was in contrast with the slower and indirect path taken by building soil structure through solid compost. Misting plants with compost tea is a more effective method for delivering nutrients thereby boosting than drenching the soil (Arancon et al., 2006; Gonález et al., 2010).
More so, vermicomposting or composting with earthworms is an excellent technique for improving the physical, chemical and biological properties of the soil. Also, use of vermicompost promotes germination growth and flowering and increases the yields of horticultural crops, tomatoes, peppers, strawberries, foliage crops, and others (Litterick et al., 2004; Ryan et al., 2005)
Consequently, altered solubility of mineral nutrients occurred and manifested as an increased content of easily available forms of microelements, heavy metals and trace elements in soil. As a result, water-based compost tea enhanced the uptake and accumulation of nutrient elements in the plant (Rodríguez-Ortíz et al., 2006). Microbial communities present in compost tea may also induce disease resistance as well as stimulate nutrient uptake and plant growth (Ingham, 2005; Scheuerell and Mahaffee, 2002).
In the study, application of aerated compost tea from organic compost based using MOVR (the mixture of rice straw compost, vermicompost, and Hinoki cypress bark compost) to the root zone increased the plant shoot and root growths and yield of the red leaf lettuce, sweet corn, and soybean. Thus, compost tea could be used as an agent for promoting plant growth in organic cultivation of crops.


This study was carried out with the support of the Research program for Agricultural Science & Technology Development (Project No. PJ00843603), funded by the Rural Development Administration, Republic of Korea in 2015.

Fig. 1
Changes in mean values of pH (A) and E.C (B) of aerated compost tea for 5 days after brewing with air pump. Error bars represent standard errors of the mean (n=4). DAT, days after treatment; OMHC, oriental medicinal herbs compost; VC, vermicomposting; RSC, rice straw compost; and MOVR, mixture of OMHC, SRC and VC.
Fig. 2
Comparison of mean values of NO3-N (A) and NH+4-N (B) of four compost tea at room temperature for 5 days after brewing with air pump. Error bars represent standard errors of the mean (n=4). DAT, days after treatment; OMHC, oriental medicinal herbs compost; VC, vermicompost; RSC, rice straw compost; MOVR, mixtures of OMHC, SRC and VC.
Fig. 3
Changes of microbial population density of aerated compost tea at room temperature for 4 days after brewing with air pump. Error bars represent standard errors of the mean (n=4). CFU, colony forming unit; DAT, days after treatment; OMHC, oriental medicinal herbs compost; VC, vermicomposting; RSC, rice straw compost; MOVR, mixture of OMHC, SRC and VC; NA, Nutrient Agar; PDA, Potato Dextrose Agar; RBPDA, PDA added Rose Bengal (60 mg/l).
Fig. 4
Major bacterial populations detected in growing compost tea based on three compost mixtures with the oriental medicinal herbs compost, rice straw compost and vermicompost (MOVR) by 16S rRNA sequencing of 100 strains. All of sequences for analyzed strains could be assigned with high confidence (≥98.5% for isolates) to known genus. The relative abundance of each genus is shown as a percentage.
Fig. 5
Growth promotion effects of the compost tea based on three compost mixtures (MOVR) with the oriental medicinal herbs compost, rice straw compost and vermicompost on soybean growth for 45 days at one week interval of foliar application of four concentration, 0.1%, 0.2%, 0.4% and 0.8% MOVR in the greenhouse.
Table 1
Growth characteristics of red leaf lettuce by different concentration of the mixture of oriental medicinal herbs compost, rice straw compost and vermicompost tea (MOVR), 0.1%, 0.2%, 0.4%, and 0.8% in greenhouse. Means followed by the same letter are not significantly different (p<0.05) within each concentration
Treatment Lettuce leaf

number length (cm) width (cm) fresh weight (g)
MOVR 0.1% 10.7 c 16.7 c 11.4 c 351.5 d
MOVR 0.2% 12.1 bc 17.5 bc 13.6 b 540.2 c
MOVR 0.4% 16.2 a 23.3 a 18.2 a 872.9 a
MOVR 0.8% 13.4 b 19.1 b 15.2 ab 653.8 b
Untreated control 10.2 c 14.0 d 10.1 d 250.2 e
Table 2
Effect of different concentration of the mixture of oriental medicinal herbs compost, rice straw compost and vermicompost tea (MOVR), 0.1%, 0.2%, 0.4%, and 0.8% in greenhouse on sweet corn growth in greenhouse. Means followed by the same letter are not significantly different (p<0.05) within each concentration
Treatment Sweet corn

Root Shoot

Length (cm) Fresh weight (g) Length (cm) Fresh weight (g)
MOVR 0.1% 4.1 d 12.8 d 20.7 d 370.5 d
MOVR 0.2% 5.2 c 14.6 c 25.0 c 420.0 c
MOVR 0.4% 6.8 b 20.1 b 30.1 b 530.8 b
MOVR 0.8% 9.1 a 24.6 a 35.3 a 720.9 a
Untreated control 2.9 e 10.8 e 16.0 e 260.2 e
Table 3
Plant responses to different concentration of the compost tea (MOVR) based on the oriental medicinal herbs compost, rice straw compost and vermicompost in the root and shoot growth and nodule number, as compared to untreated control at 45 days old soybean plant in greenhouse. Means followed by the same letter are not significantly different (p<0.05) within each concentration
Treatment Soybean

Root Shoot Nodule number

length (cm) weight (g) height (cm) fresh weight (g)
MOVR 0.1% 12.9 d 18.7 c 20.8 c 81.7 d 23.4 d
MOVR 0.2% 15.6 c 23.0 b 24.5 b 97.3 c 42.1 c
MOVR 0.4% 19.1 b 23.5 b 26.9 b 129.6 b 106.3 b
MOVR 0.8% 24.1 a 53.5 a 30.9 a 157.1 a 164.5 a
Untreated control 8.0 e 14.2 d 14.4 d 63.6 e 5.4 e


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