Plant Pathol J > Volume 24(2); 2008 > Article
The Plant Pathology Journal 2008;24(2):131-142.
DOI: https://doi.org/10.5423/PPJ.2008.24.2.131    Published online June 30, 2008.
Identification of Genes Encoding Heat Shock Protein 40 Family and the Functional Characterization of Two Hsp40s, MHF16 and MHF21, in Magnaporthe oryzae
Mi Hwa Yi, Yong Hwan Lee
Abstract
Magnaporthe oryzae, the causal agent of the rice blast disease, poses a worldwide threat to stable rice production. The large-scale functional characterization of genes controlling the pathogenicity of M. oryzae is currently under way, but little is known about heat shock protein 40 (Hsp40) function in the rice blast fungus or any other filamentous plant pathogen. We identified 25 genes encoding putative Hsp40s in the genome of M. oryzae using a bioinformatic approach, which we designated M. oryzae heat shock protein forty (MHF1-25). To elucidate the roles of these genes, we characterized the functions of MHF16 and MHF21, which encode type III and type II Hsp40 proteins, respectively. MHF16 and MHF21 expression was not significantly induced by heat shock, but it was downregulated by cold shock. Knockout mutants of these genes (Δmhf16 and Δmhf21) were viable, but conidiation was severely reduced. Moreover, sectoring was observed in the Δmhf16 mutant when it was grown on oatmeal agar medium. Conidial germination, appressorium formation, and pathogenicity in rice were not significantly affected in the mutants. The defects in conidiation and colony morphology were fully complemented by reintroduction of wild type MHF16 and MHF21 alleles, respectively. These data indicate that MHF16 and MHF21 play important roles in conidiation in the rice blast fungus.
Key Words: conidiation, heat shock protein 40, MHF, sectoring
TOOLS
METRICS Graph View
  • 6 Crossref
  •  0 Scopus
  • 703 View
  • 5 Download
Related articles


ABOUT
BROWSE ARTICLES
EDITORIAL POLICY
FOR CONTRIBUTORS
Editorial Office
Rm,904 (New Bldg.) The Korean Science & Technology Center 22,
Teheran-ro 7-Gil, Gangnamgu, Seoul 06130, Korea
Tel: +82-2-557-9360    Fax: +82-2-557-9361    E-mail: paper@kspp.org                

Copyright © 2024 by Korean Society of Plant Pathology.

Developed in M2PI

Close layer
prev next