Plant Pathol J > Volume 24(3); 2008 > Article
The Plant Pathology Journal 2008;24(3):309-316.
DOI:    Published online September 30, 2008.
Biocontrol of Blue Stain in Pine Wood with Lyophilized Mycelium of Ophiostoma quercus Albino Strain
Byung Ju Cho, Nam Kyu Kim, Nam Seok Cho, Jong Kyu Lee
Mycelium of Ophiostoma quercus albino strain cultured in liquid culture media was harvested, lyophilized, and stored for examining biocontrol efficacy against wood discoloration by staining fungi in the laboratory and field conditions. Dry weight of mycelium grown in brown sugar yeast extract broth (BYB) showed 3.8 times higher than that grown in potato dextrose broth (PDB). The optimum culture period in BYB was 4 weeks. In vitality test of the albino strain, the lyophilized mycelium stored in liquid nitrogen (-196oC) or in a refrigerator (4oC) kept the vitality until 13 months after storage; however, the mycelium stored at room temperature lost the vitality completely after 13 months. The mycelium stored in liquid nitrogen or in a refrigerator protected wood chips from the discoloration by pretreating mycelial suspension on pine wood chips. The mycelium stored at room temperature for 7 months also showed complete protection. These results suggest that the lyophilized mycelium have a biocontrol efficacy only if it keeps the least vitality. In the field conditions, both albino strain and Woodguard
Key Words: biocontrol, blue stain fungi, Cartapip

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