Plant Pathol J > Volume 25(1); 2009 > Article
The Plant Pathology Journal 2009;25(1):77-85.
DOI: https://doi.org/10.5423/PPJ.2009.25.1.077    Published online March 31, 2009.
Identification of a Genetic Locus Related to Antivirus Production in Pseudomonas fluorescence strain Gpf01 Against Cucumber mosaic virus
Sae Youll Cho, Seon Hwa Lee, Su Jin Park, Kyu Up Choi, Jun Mo Cho, Jang Hyun Hur, Anupama Shrestha, Chun Keun Lim
Abstract
Pseudomonas fluorescens strain Gpf01, isolated from ginseng rhizosphere showed antiviral activity against Cucumber mosaic virus, when tested in a local host of CMV, Chenopodium amaranticolor. Transposon mutant library of Gpf01 was prepared using pGS9::Tn5 and the mutant Gpf01-RS19 was found to loose antiviral production. We developed primers from the flanking region of Tn5 and found a cosmid clone pAV1123, harboring 1.2 kb antiviral compound producing (avcf01) locus. When a sub-clone pPH9, which carried 9.3 kb region of pAV1123, was introduced into antivirus deficient P. fluorescens wild type strain B16, it exhibited antiviral activity. Using Tn3-gus mutagenesis and complementation analysis, it was found that the genes related to antiviral activity production resided in a 9.3- kb HindIII-HindIII fragment of pAV1123, indicating that the plasmid carries an essential genes promoting antiviral activity.
Key Words: antiviral compound producing gene, Cucumber mosaic virus, Pseudomonas fluorescens
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