Molecular Analysis of Korean Isolate of Barley mild mosaic virus (Iks Isolate)

Choi, Min Kyung; Kannan, Seralathan Kamala; Oh, Byung Taek; Park, Jong Chul; Lee, Gun Woong; Lee, Kui Jae; Park, Yool Jin

The Plant Pathology Journal 2009;25(2):157-164.
Published online June 30, 2009.

Molecular Analysis of Korean Isolate of Barley mild mosaic virus (Iks Isolate)

Min Kyung Choi, Seralathan Kamala Kannan, Byung Taek Oh, Jong Chul Park, Gun Woong Lee, Kui Jae Lee, Yool Jin Park

Abstract

The complete nucleotide sequences of both RNA of an isolated Barley mild mosaic virus (BaMMV) from Iksan, Korea, have been determined. RNA1 was 7273 nucleotides long and encodes for a polyprotein of 2261 amino acids, which contains the eight putative functional proteins. RNA2 was 3520 nucleotides long and encodes for a polyprotein of 894 amino acids, which contains two functional proteins. Results of multiple sequence alignment showed 92.9% similarity with Na1 isolate, followed by Sil, UK(F), Asl1, Remis M and UK (M) isolates, respectively. Comparison of the BaMMVIks polyproteins with the corresponding proteins of BaMMV-Na1 isolates showed 95% amino acid sequence identity. The phylogenetic analysis revealed that Iks isolate was closely related to Na1 strain and have a common origin.

Key Words: Barley mild mosaic virus, capsid protein, fungus transmission, Hordeum vulgare, Potyvir