The Plant Pathology Journal 2009;25(4):369-375.
Published online December 31, 2009.
Antiviral Effects of the Culture Filtrate from Serratia marcescens Gsm01, against Cucumber mosaic virus (CMV)
Shree Prasad Thapa, Hye Jin Lee, Duck Hwan Park, Sam Kyu Kim, Jun Mo Cho, Sae Youll Cho, Jang Hyun Hur, Chun Keun Lim
The potential antiviral effects of the culture filtrates (CF) from Serratia marcescens strain Gsm01 against yellow strain of Cucumber mosaic virus (CMV-Y) were investigated. The culture filtrate of S. marcescens strain Gsm01 applied on Chenopodium amaranticolor showed high inhibitory activity, likewise no necrosis appeared when applied on the tobacco plants 2 days before CMVY inoculation. When plants were challenge inoculated with CMV-Y for eighteen days, the disease incidence in plants with culture filtrate of S. marcescens Gsm01 did not exceed 59%, whereas 100% of control plants were severely infected. The results of double antibody sandwich- enzyme linked immunosorbent assay (DAS-ELISA), reverse transcriptase polymerase chain reaction (RTPCR), dot blotting, and western blotting showed that culture filtrate treatment highly affected the accumulation of CMV-Y or its CP protein gene in the treated plant leaves. It was also observed that the culture filtrate had no RNase activity on genomic RNAs of CMV-Y, suggesting that culture filtrate may not contain ribosome inactivating proteins (RIPs) or proteins with RNase activity. These data shows that culture filtrate of S. marcescens strain Gsm01 seems to be a promising source of antiviral substance for the practical use.
Key Words: culture filtrate, Cucumber mosaic virus, Serratia marcescens

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