Plant Pathol J > Volume 25(4); 2009 > Article
The Plant Pathology Journal 2009;25(4):400-407.
DOI:    Published online December 31, 2009.
Isolation and Characterization of Pathogenesis-Related Protein 5 (PgPR5) Gene from Panax ginseng
Yu Jin Kim, Jung Hye Lee, Dae Young Jung, Gayathri Sathiyaraj, Ju Sun Shim, Jun Gyo In, Deok Chun Yang
A pathogenesis-related protein (PgPR5) gene that isolated from the leaf of Panax ginseng was characterized. The ORF is 756 bp with a deduced amino acid sequence of 251 residues. The calculated molecular mass of the matured protein is approximately 27.5 kDa with a predicated isoelectric point of 7.80. A GenBank BlastX search revealed that the deduced amino acid of PgPR5 shares highest sequence similarity to PR5 of Actinidia deliciosa (80% identity, 87% similarity). PgPR5 has a Cterminal and N-terminal signal peptide, suggesting that it is a vacuolar secreted protein. The expression of PgPR5 under various environmental stresses was analyzed at different time points using real-time PCR. Our results reveal that PgPR5 is induced by salt stress, chilling stress, heavy metal, UV, and pathogen infection. These results suggest that the PgPR5 could play a role in the molecular defence response of ginseng to abiotic and pathogen attack. This is the first report of the isolation of PR5 gene from the P. ginseng.
Key Words: biotic stress, chilling, ginseng, pathogenesisrelated protein5, salt stress

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