Species-specific Detection of Erwinia pyrifoliae by PCR Assay Using Enterobacterial Repetivive Intergenic Consensus (ERIC) Primers |
Duck Hwan Park, Shree Prasad Thapa, Won Sik Kim, Jang Hyun Hur, Chun Keun Lim |
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Abstract |
We designed a sensitive and specific PCR-based method with enterobacterial repetitive intergenic consensus (ERIC) primer to detect Erwinia pyrifoliae, which cause shoot blight in Asian pear, from a mixed culture and infected plant materials. The primers specifically detected only E. pyrifoliae and showed no cross-reactivity with other bacterial phytopathogens. |
Key Words:
detection, Erwinia pyrifoliae, ERIC, Pyrus pyrifolia |
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