Plant Pathol J > Volume 26(3); 2010 > Article
The Plant Pathology Journal 2010;26(3):267-270.
DOI: https://doi.org/10.5423/PPJ.2010.26.3.267    Published online September 30, 2010.
Species-specific Detection of Erwinia pyrifoliae by PCR Assay Using Enterobacterial Repetivive Intergenic Consensus (ERIC) Primers
Duck Hwan Park, Shree Prasad Thapa, Won Sik Kim, Jang Hyun Hur, Chun Keun Lim
Abstract
We designed a sensitive and specific PCR-based method with enterobacterial repetitive intergenic consensus (ERIC) primer to detect Erwinia pyrifoliae, which cause shoot blight in Asian pear, from a mixed culture and infected plant materials. The primers specifically detected only E. pyrifoliae and showed no cross-reactivity with other bacterial phytopathogens.
Key Words: detection, Erwinia pyrifoliae, ERIC, Pyrus pyrifolia
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