The Plant Pathology Journal 2010;26(3):286-288.
Published online September 30, 2010.
Microwave Irradiation-assisted RNA Extraction from Woody Tissues for Plant Virus Detection
Thanh Van Duong, Dong Il Shin, Hee Sung Park
Abstract
Plant tissue homogenization using a mortar or mechanical equipment has been the preferred method for obtaining high yields of total RNA; this method, however, is both time-consuming and expensive. Additionally, homogenization may generate excessive endogenous RNases, polyphenolics, and other substances that reduce the quality and quantity of RNA. In this study, we describe the microwave irradiation-assisted RNA extraction (MIRE) technique which, without tissue disruption and homogenization, allows for the cost-effective and rapid generation of intact RNA from apple cane shavings and the reliable detection of apple virus by RT-PCR.
Key Words: microwave irradiation, RNA extraction, RTPCR, plant virus
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