The Plant Pathology Journal 2010;26(3):289-292.
Published online September 30, 2010.
Simple and Reliable DNA Extraction Method for the Dark Pigmented Fungus, Cercospora sojina
Ji Seong Kim, Sang Gyu Seo, Byung Ki Jun, Jin Won Kim, Sun Hyung Kim
Abstract
This study used a modified cetyltrimethylammonium bromide (CTAB) method to efficiently extract DNA from the plant pathogenic fungus Cercospora sojina. Total DNA yield obtained by this method was approximately 1 mg/g of mycelia (fresh weight), and the mean ratio of A260/A280 and A260/A230 were 2.04 and 2.1, respectively. The results of random amplified polymorphic DNA (RAPD) analysis, digestion with restriction enzymes, and Southern hybridization indicated that polysaccharides were effectively removed by this method, and the resulting DNA was sufficient for use in subsequent molecular analysis.
Key Words: Cercospora sojina, DNA extraction, fungus, RAPD, Restriction enzyme
TOOLS
METRICS Graph View
  • 401 View
  • 6 Download
Related articles


ABOUT
BROWSE ARTICLES
EDITORIAL POLICY
FOR CONTRIBUTORS
Editorial Office
Rm,904 (New Bldg.) The Korean Science & Technology Center 22,
Teheran-ro 7-Gil, Gangnamgu, Seoul 06130, Korea
Tel: +82-2-557-9360    Fax: +82-2-557-9361    E-mail: paper@kspp.org                

Copyright © 2023 by Korean Society of Plant Pathology.

Developed in M2PI

Close layer
prev next