Morphology, Molecular Phylogeny and Pathogenicity of Colletotrichum panacicola Causing Anthracnose of Korean Ginseng

Choi, Kyung Jin; Kim, Wan Gyu; Kim, Hong Gi; Choi, Hyo Won; Lee, Young Kee; Lee, Byung Dae; Lee, Sang Yeob; Hong, Sung Kee

doi:2011.27.1.1

Plant Pathol J > Volume 27(1); 2011 > Article

The Plant Pathology Journal 2011;27(1):1-7.
DOI: https://doi.org/10.5423/PPJ.2011.27.1.001 Published online March 31, 2011.

Morphology, Molecular Phylogeny and Pathogenicity of Colletotrichum panacicola Causing Anthracnose of Korean Ginseng

Kyung Jin Choi, Wan Gyu Kim, Hong Gi Kim, Hyo Won Choi, Young Kee Lee, Byung Dae Lee, Sang Yeob Lee, Sung Kee Hong

Abstract

Colletotrichum panacicola isolates were obtained from anthracnose lesions of Korean ginseng and compared with four Colletotrichum species in morphology, molecular phylogeny and pathogenicity. Based on morphological characteristics, C. panacicola was easily distinguished from Colletotrichum gloeosporioides but not from Colletotrichum higginsianum, Colletotrichum destructivum and Colletotrichum coccodes. A phylogenetic tree generated from ribosomal DNA-internal transcribed spacer sequences revealed that C. panacicola is remarkably distinguished from C. gloeosporioides and C. coccodes but not from C. higginsianum and C. destructivum. However, molecular sequence analysis of three combined genes (actin + elongation factor-1α + glutamine synthatase) provided sufficient variability to distinguish C. panacicola from other Colletotrichum species. Pathogencity tests showed that C. panacicola is pathogenic to Korean ginseng but not to other plants. These results suggest that C. panacicola is an independent taxon distin-zguishable from C. gloeosporioides and other morphologically similar Colletotrichum species.

Key Words: Anthracnose, Colletotrichum gloeosporioides, Colletotrichum panacicola, Korean ginseng