Strawberry mild yellow edge potexvirus from Strawberry in Korea

Cho, Jeom Deog; Choi, Gug Seoun; Chung, Bong Nam; Kim, Jeong Soo; Choi, Hong Soo

The Plant Pathology Journal 2011;27(2):187-190.
Published online June 30, 2011.

Strawberry mild yellow edge potexvirus from Strawberry in Korea

Jeom Deog Cho, Gug Seoun Choi, Bong Nam Chung, Jeong Soo Kim, Hong Soo Choi

Abstract

Symptoms induced in the leaves of strawberry (Fragaria x ananassa Duch.), ``Seolhyang`` and ``Eyeberry``, were mosaic, distortion and black colored edge on leaves at Nonsan area, one of the important production areas in Korea. Electron microscopy by quick-dip revealed the flexuous rod-shape particles having about 550-600 nm length. Cytoplasmic inclusion bodies composed of aggregated virus particles were observed frequently in mesophyll parenchyma and epidermal cells for the leaves of strawberry. The specific primers amplifying products of 635 bp and 729 bp were developed for RT-PCR detection of Strawberry mild yellow edge virus (SMYEV). Nucleotide identity of the CP gene of SMYEV was 92.8- 99.2% with those of other SMYEV isolates from Gen- Bank database.

Key Words: nucleotide identity, RT-PCR, SMYEV, strawberry, ultrastructure