The Plant Pathology Journal 2012;28(2):197-201.
Published online June 30, 2012.
Characterization of the in vitro Activities of the P1 and Helper Component Proteases of Soybean mosaic virus Strain G2 and Tobacco vein mottling virus
Hyoun Sub Lim, Chan Yong Jang, Ji Ryun Nam, Mei Jia Li, Jin Sung Hong, Han Hong Bae, Ho Jong Ju, Hong Gi Kim, Richard E. Ford, Leslie L. Domier
Abstract
Potyviruses express their RNA genomes through the production of polyproteins that are processed in host cells by three virus-encoded proteases. Soybean plants produce large amounts of protease inhibitors during seed development and in response to wounding that could affect the activities of these proteases. The in vitro activities of two of the proteases of Soybean mosaic virus (SMV) and Tobacco vein mottling virus (TVMV) were compared in the rabbit reticulocyte lysate in vitro translation system using synthetic RNA transcripts. Transcripts produced from SMV and TVMV cDNAs that included the P1 and helper component-protease (HCPro) coding regions directed synthesis of protein products that were only partially processed. Unprocessed polyproteins were not detected from transcripts that included all of the P1, HC-Pro, P3 and portions of the cylindrical inclusion protein coding regions of either virus. Addition of soybean trypsin inhibitor to in vitro translation reactions increased the accumulation of the unprocessed polyprotein from TVMV transcripts, but did not alter the patterns of proteins produced from SMV. These experiments suggest that SMV- and TVMVencoded proteases are differentially sensitive to protease inhibitors.
Key Words: Helper-component protease, in vitro translation, P1 protease, trypsin inhibitor
TOOLS
METRICS Graph View
  • 519 View
  • 2 Download
Related articles

Complete Genome Sequences of the Genomic RNA of Soybean mosaic virus Stranins G7H and G52003 June;19(3)



ABOUT
BROWSE ARTICLES
EDITORIAL POLICY
FOR CONTRIBUTORS
Editorial Office
Rm,904 (New Bldg.) The Korean Science & Technology Center 22,
Teheran-ro 7-Gil, Gangnamgu, Seoul 06130, Korea
Tel: +82-2-557-9360    Fax: +82-2-557-9361    E-mail: paper@kspp.org                

Copyright © 2023 by Korean Society of Plant Pathology.

Developed in M2PI

Close layer
prev next