Research Articles : Biocontrol Potential of Streptomyces griseus H7602 Against Root Rot Disease (Phytophthora capsici) in Pepper

Nguyen, Xuan Hoa; Naing, Kyaw Wai; Lee, Young Seong; Tindwa,  Hamisi; Lee, Geon Hyoung; Jeong, Byoung Kon; Ro, Hee Myeong; Kim, Sang Jun; Jung, Woo Jin; Kim, Kil Yong

The Plant Pathology Journal 2012;28(3):282-289.
Published online September 30, 2012.

Research Articles : Biocontrol Potential of Streptomyces griseus H7602 Against Root Rot Disease (Phytophthora capsici) in Pepper

Xuan Hoa Nguyen, Kyaw Wai Naing, Young Seong Lee, Hamisi Tindwa, Geon Hyoung Lee, Byoung Kon Jeong, Hee Myeong Ro, Sang Jun Kim, Woo Jin Jung, Kil Yong Kim

Abstract

The root rot of pepper (Capsicum annuum L.) caused by Phytophthora capsici is one of the most important diseases affecting this crop worldwide. This work presents the evaluation of the capacity of Streptomyces griseus H7602 to protect pepper plants against Phytophthora capsici and establishes its role as a biocontrol agent. In this study, we isolated an actinomycete strain H7602 from rhizosphere soil, identified it as Streptomyces griseus by 16S rRNA analysis and demonstrated its antifungal activity against various plant pathogens including P. capsici. H7602 produced lytic emzymes such as chitinase, β-1,3-glucanase, lipase and protease. In addition, crude extract from H7602 also exhibited destructive activity toward P. capsici hyphae. In the pot trial, results showed the protective effect of H7602 against pepper from P. capsici. Application of H7602 culture suspension reduced 47.35% of root mortality and enhanced growth of pepper plants for 56.37% in fresh root and 17.56% g in fresh shoot as compared to control, resulting in greater protection to pepper plants against P. capsici infestation. Additionally, the enzymatic activities, chitinase and β- 1,3-glucanase, were higher in rhizosphere soil and roots of pepper plants treated with H7602 than other treated plants. Therefore, our results indicated a clear potential of S. griseus H7602 to be used for biocontrol of root rot disease caused by P. capsici in pepper.

Key Words: antifungal activity, biocontrol, Phytophthora capsici, Streptomyces griseus