| Research Notes : Characterization of a Novel Cucumber mosaic virus Isolated from Petunia hybrida |
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Kyung Sook Han, Gug Seoun Choi, Bong Nam Chung, Jeom Duk Cho, In Sook Cho, Kee Hong Kim, Su Kim, Ju Yeon Yoon, Seung Kook Choi |
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| Abstract |
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Petunia hybrida is commonly used in landscapes and interiors for its attractive flower. Virus-like foliar symptoms, including a mosaic with dark green islands surrounding the veins and chlorosis on the leaf margins, were observed on a petunia plant from Icheon, Gyeonggido, Korea. Cucumber mosaic virus (CMV) was identified in the symptomatic petunia by serological testing for the presence of CMV coat protein (CP) with a direct antibody-sandwich-enzyme-linked immunosorbent assay. An agent was mechanically transmitted to indicator plant species including Chenopodium quinoa. Examination of the inoculated plant leaves by RT-PCR analysis and electron microscopy revealed the presence of specifically amplified CP products and spherical virions of approximately 28 nm in diameter, respectively, providing confirmation of a CMV infection. Analysis of CP sequences showed that CMV petunia isolate (CMVYJC) shared 82.5-100% amino acid sequence identity with CPs of representative CMV strains. Phylogenetic analysis of CPs supports that CMV-YJC is a member of CMV subgroup IA (CMV-IA) and has biological properties of CMV-IA on host species. To our knowledge, this is the first report of CMV from P. hybrida in Korea. |
| Key Words:
coat protein, Cucumber mosaic virus, petunia, RT-PCR, sequence analysis, symptom |
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