In Vitro and In Vivo Inhibitory Effects of Gaseous Chlorine Dioxide against Fusarium oxysporum f. sp. batatas Isolated from Stored Sweetpotato: Study II
Article information
Abstract
Chlorine dioxide (ClO2) has been widely used as an effective disinfectant to control fungal contamination during postharvest crop storage. In this study, Fusarium oxysporum f. sp. batatas SP-f6 from the black rot symptom of sweetpotato was isolated and identified using phylogenetic analysis of elongation factor 1-α gene; we further examined the in vitro and in vivo inhibitory activities of ClO2 gas against the fungus. In the in vitro medium tests, fungal population was significantly inhibited upon increasing the concentration and exposure time. In in vivo tests, spore suspensions were drop-inoculated onto sweetpotato slices, followed by treatment using various ClO2 concentrations and treatment times to assess fungus-induced disease development in the slices. Lesion diameters decreased at the tested ClO2 concentrations over time. When sweetpotato roots were dip-inoculated in spore suspensions prior to treatment with 20 and 40 ppm of ClO2 for 0–60 min, fungal populations significantly decreased at the tested concentrations for 30–60 min. Taken together, these results showed that ClO2 gas can effectively inhibit fungal growth and disease development caused by F. oxysporum f. sp. batatas on sweetpotato. Therefore, ClO2 gas may be used as a sanitizer to control this fungus during postharvest storage of sweetpotato.
Chlorine has been widely applied to control postharvest diseases in various fruits and vegetables such as blueberries, cucumber, oranges, potatoes, strawberries, and tomatoes (Bhagat et al., 2011; Mahovic et al., 2007; Popa et al., 2007; Tweddell et al., 2003; Yuk et al., 2006). Chlorine has a strong oxidation activity on cellular constituents and combines directly with membrane proteins and enzymes (Tweddell et al., 2003). Chlorine, with these action traits, can destroy various microbes existing on fruits and vegetables. Owing to this ability, chlorine dioxide (ClO2) could be used as an alternative sanitizer as its oxidation capacity is 2.5 times higher than that of chlorine (Beuchat et al., 2004). ClO2 works effectively and rapidly against microbes at low concentrations while its toxicity to human and animals is considered low (Vaid et al., 2010; Wang et al., 2016). Thus, ClO2 as a sanitizer has been approved for agricultural and medical uses by the United States Environmental Protection Agency (EPA) (2006). Recently, the application of gaseous ClO2 (e.g., ClO2 concentration, time, and temperature) against pathogenic microbes on postharvest stage and storage of various fruits and vegetables was well documented by Sun et al. (2019).
Sweetpotato (Ipomoea batatas Lam.) is one of the most important food crops worldwide (Sanusi et al., 2016). While the consumption of sweetpotato has increased, postharvest diseases (especially fungal contamination) during crop storage are significant limitations (estimated quantity and quality loss, 15–65%) (Ray and Ravi, 2005). Sweetpotato is generally stored in storage houses, in which fungal contamination may spread throughout the storage facility, resulting in significant contamination of the stored crop (Wu and Rioux, 2010). Hence, it is important to apply effective control measures to prevent fungal infection during sweetpotato storage. Fungicides such as thiabendazole, dichloran, benomyl, and iprodione may be used to control root rots of sweetpotato in fields or to obtain disease-free ‘seed’ roots for plant production (Clark et al., 2013). However, application of agricultural chemicals is generally avoided during sweetpotato storage because the crop roots are directly used as a food source. Thus, ClO2 gas, as described above, may be used as an alternative measure to control fungal contamination during crop storage.
Numerous Fusarium spp. have been reported to infect sweetpotato roots (Farr and Rossman, 2018); however, Fusarium wilt caused by Fusarium oxysporum f. sp. batatas is one of the major diseases of sweetpotato (Clark et al., 2013). Fusarium wilt is usually widespread and shows higher infection levels in commercial fields (Thompson et al., 2011). In Korea, this disease was first reported by Park (1967) and has caused significant economic losses in sweetpotato production areas such as Haenam. In general, Fusarium wilt symptoms include yellowing of older leaves followed by wilting, abscission of older leaves, and stunting of vine growth (Clark et al., 2013). The infection of sweetpotato plants by the pathogen can occur from soil-borne inoculum such as chlamydospores. Thus, soil as a source of inoculum may also contribute to the infection of sweetpotato roots at harvest and root rot may follow during storage. In this sense, it is important to find measures to limit infection of the pathogen to sweetpotato roots (Clark et al., 2013).
Previously, we often observed black rot symptoms on stored sweetpotato roots and frequently isolated Fusarium spp. from the infected roots. We suspected the causal agent for this symptom to be the pathogen causing Fusarium wilt on sweetpotato plants in fields. Therefore, the objectives of this study were (1) to identify the causal agent of the black rot symptom of stored sweetpotato roots and (2) to evaluate in vitro and in vivo inhibitory activities of gaseous ClO2 against the causal agent isolated from sweetpotato at various ClO2 concentrations and treatment times.
Materials and Methods
Fungus and sweetpotato
Diseased sweetpotato roots (cv. ‘Juwhangmi’) supplied from the Bioenergy Crop Research Center, National Institute of Crop Science, Rural Development Administration (Muan, Korea) in August 2015 were used in this study (Fig. 1A and B). For fungal isolation, small pieces of tissues from the margins of the diseased root were cut and sterilized with 1% NaOCl for 2 min. The tissue pieces were then washed with sterile distilled water three times and blotted on a sterile filter paper (Whatman No. 1). The pieces were placed on acidified potato dextrose agar (PDA) supplemented with lactic acid (600 μl/l) at 25°C. Two to three days after placement on the medium, hyphal tips of mycelia grown from tissue pieces (one per lesion) were transferred to acidified PDA. Obtained isolates were further stored on PDA slants at 4°C until use. A representative isolate SP-f6 from isolated cultures was selected and subjected to further identification. Isolate SP-f6 was grown on Spezieller Nährstoffarmer agar (SNA) for macroconidium and microconidium formation (Leslie and Summerell, 2006). Healthy sweetpotato roots (cv. ‘Juwhangmi’) from the same center mentioned above were used in this study and these were stored in a moist chamber with 60% relative humidity at 15°C until use.
Molecular identification of isolate SP-f6
For molecular identification of isolate SP-f6 obtained from the diseased root tissue, the genomic DNA was extracted from fungal mycelia grown on PDA at 25°C for 7 days using an i-genomic BYF DNA Extraction Kit (iNtRON Biotechnology, Seongnam, Korea) according to the manufacturer’s instructions. The internal transcribed spacer (ITS) regions of isolate SP-f6 were amplified using the universal primers ITS1 (5′-TCCGTAGGTGAACCTGCGG-3′) and ITS4 (5′-TCCTCCGCTTATTGATATGC-3′) (Glass and Donaldson, 1995); the elongation factor 1-α gene of the isolate was amplified with primers EF-1 (5′-ATGGGTAAGGAAGACAAGAC-3′) and EF-2 (5′-GGAAGTACCAGTGATCATGTT-3′) (Kim et al., 2005). Polymerase chain reaction and DNA sequence analysis were conducted as described by Kim et al. (2009) and Sang et al. (2013). The sequenced data were analyzed using the Basic Local Alignment Search Tool (BLAST) at the NCBI website (https://blast.ncbi.nlm.nih.gov/Blast.cgi). A phylogenetic tree was constructed using the neighbor-joining (Saitou and Nei, 1987) and maximum-likelihood (Fitch, 1971) algorithms using Molecular Evolutionary Genetics Analysis (MEGA) version 6.0. The topology of the tree was assessed by analyzing bootstrap with 1000 replications (Felsenstein, 1985). The experiments were performed twice for confirmation.
Pathogenicity test of isolate SP-f6
For pathogenicity tests of isolate SP-f6, healthy sweetpotato roots (cv. ‘Juwhangmi’) were washed with tap water and rinsed with distilled water. The roots were surface-sterilized with 70% ethanol for 5 min. Then, these roots were rinsed with distilled water twice and dried for 10 min at 15°C. The roots were hole-inoculated with isolate SP-f6 grown on PDA at 25°C for 7 days. Holes in roots were made using a sterile 4-mm cork-borer. Mycelial plugs (5 mm in diameter) from the edges of the growing cultures of isolate SP-f6 or PDA plugs (uninoculated control) were inoculated into holes made in the outer layers of the sweetpotato roots [six holes: three (sub-replicates) for SP-f6 and three for PDA plug per root (replicate)]. The inoculated roots were then wrapped up in polyethylene film (Cleanwrap Co., Seoul, Korea) and placed in moist plastic containers [23 (length) × 13 (width) × 16 (height) cm] with three layers of wet paper towels (100% RH) and incubated at 28°C. After 10 days of incubation, lesion diameters (mm) were measured. At the same time, fungus was re-isolated from the symptomatic tissues to fulfill Koch’s postulates. The edges of lesions were cut, sterilized with 1% NaOCl for 1.5–5 min, washed three times with sterile distilled water, and blotted on a sterile filter paper (Whatman No. 1). These pieces were placed on acidified PDA at 25°C for 3 days. The mycelia grown from the pieces was transferred to PDA and the identity of the isolate reconfirmed by the elongation factor 1-α gene sequence analysis, as described above.
In vitro effect of gaseous chlorine dioxide against isolate SP-f6 on medium
To examine the in vitro inhibitory effect of gaseous ClO2 against isolate SP-f6 on the medium, spore suspensions (200 μl) of the isolate were spread on acidified PDA. Spores from cultures of isolate SP-f6 grown on PDA at 25°C for 7 days were harvested with 0.03% Tween 20 and adjusted to 5 × 103 spores/ml using a hemocytometer. Next, these spore-inoculated plates were treated with various concentrations (1, 5, 10, and 20 ppm) of ClO2 gas for 0, 1, 10, 30, and 60 min. The gas treatment for various treatment times was conducted using a ClO2 generator (PurgoFarm Co. Ltd., Hwaseong, Korea) with an electrochemical method (Gates, 1998), as described in our previous study (Lee et al., 2019). The gas-treated plates were further incubated at 25°C. After 2 days of incubation, numbers of colony-forming units (CFUs) on the plates were counted.
In vivo effect of gaseous chlorine dioxide against isolate SP-f6 on root slices
To examine the in vivo inhibitory effect of gaseous ClO2 against isolate SP-f6 on root slices, healthy sweetpotato roots prepared as described in the pathogenicity test were cut into 1-cm-thick slices, and placed in Petri plates (90 mm in diameter). The centers of the slices were drop-inoculated with the spore suspension (10 μl of 5 × 106 spores/ml) prepared as described above. The inoculated slices were treated with various concentrations (5, 10, and 20 ppm) of ClO2 gas for 0, 10, 30, and 60 min, as described above. Next, the gas-treated slices were put in the plastic containers containing wet paper towels as described above and incubated at 28°C for 14 days. Then, lesion diameters on the gas-treated, inoculated slices were assessed as follows: longest lesion length (mm) + shortest lesion length (mm) were divided by two.
In vivo effect of gaseous chlorine dioxide against isolate SP-f6 on sweetpotato roots
To examine the in vivo inhibitory effect of gaseous ClO2 against isolate SP-f6 on sweetpotato, roots were dipped in spore suspension (5 × 106 spores/ml) prepared as described above, for 10 min. The 0.03% Tween 20 was used as an uninoculated control. The inoculated roots were treated with different concentrations (20 and 40 ppm) of ClO2 gas for 0, 30, and 60 min as described above. Then, the surface layers (approximately 1.5 mm in thickness) of the gas-treated roots were peeled using a vegetable peeler. Samples (2 g) were finely ground using an analytical mill (IKA A11 basic, IKA Works) in sterile distilled water (20 ml). After serial dilutions of the ground sample solution, the samples were spread on acidified PDA and further incubated at 25°C. After 2 days of incubation, colonies that appeared on the medium were counted; these were expressed as CFUs per g dry weight.
Statistical analysis
Experiments were conducted using factorial designs to recognize the effects of ClO2 gas concentration and treatment time with various levels on lesion diameters and fungal populations. All experiments except molecular identification of the isolate were conducted twice with three replicates per treatment. Fungal population data from repeated experiments of the in vitro ClO2 inhibitory tests on the medium were pooled after confirmation of homogeneity of variances, using Levene’s test (Levene, 1960). In addition, fungal population data in this study were analyzed after logarithmic transformation. Analysis of variance (ANOVA) was conducted using general linear model procedures; the means were separated using least significant difference (LSD) test at P < 0.05. Statistical analysis of all data was conducted using Statistical Analysis Systems (SAS) software (SAS Institute, Cary, NC, USA).
Results
Fungal isolation and identification of isolate SP-f6
The potential causal fungus was consistently isolated from the margins of symptomatic lesions of diseased sweetpotato roots. Isolate SP-f6 randomly selected from isolates obtained from diseased sweetpotato roots (cv. Juwhangmi) showed white floccose mycelium and pink to purple reverse color (Fig. 1C and D); consequently, it was considered as the genus Fusarium. In addition, macroconidia and microconidia of isolate SP-f6 were observed on SNA medium (Fig. 1E).
For the phylogenetic analysis of isolate SP-f6, partial sequences (469 and 608 bases) of the ITS region and elongation factor 1-α alpha genes, respectively, were analyzed. In the neighbor-joining analysis using the ITS region sequence, isolate SP-f6 was clustered with Fusarium oxysporum CBS 132475 (100% similarity), Fusarium inflexum NRRL 20433T (99.28% similarity), F. oxysporum f. sp. batatas CBS 258.50 (98.08% similarity), and F. oxysporum f. sp. batatas CBS 172.30 (98.08% similarity) (Supplementary Fig. 1). On the other hand, based on the neighbor-joining analysis of elongation factor 1-α gene, isolate SP-f6 was clustered with F. oxysporum f. sp. batatas NRRL 22535 (100% similarity), F. oxysporum f. sp. batatas NRRL 36135 (99.84% similarity), F. oxysporum f. sp. batatas NRRL 36391 (98.84% similarity), Fusarium inflexum NRRL 20433T (99.04 similarity), and Fusarium oxysporum CBS 132475 (99.04 similarity) (Fig. 2). Thus, isolate SP-f6 was clearly identified as F. oxysporum f. sp. batatas based on the analysis of elongation factor 1-α gene sequence. The ITS (MK212928) and the elongation factor 1-α gene (MK358411) sequences of F. oxysporum f. sp. batatas SP-f6 have been deposited in GenBank.
Pathogenicity of F. oxysporum f. sp. batatas SP-f6 on sweetpotato
In the pathogenicity test, F. oxysporum f. sp. batatas SP-f6 caused dark grayish round lesions with white mycelia on sweetpotato (cv. Juwhangmi) roots 10 days after inoculation (Fig. 3A). Lesion diameters on outer layers of roots inoculated with isolate SP-f6 were 8.72 ± 0.70 mm (mean ± standard error) and 10.22 ± 0.49 mm in repeated experiments. However, PDA plugs (uninoculated control) did not produce any symptoms on the root (Fig. 3A). To fulfill Koch’s postulates, isolate SP-f6 was isolated again from the symptomatic tissues of the inoculated roots, in which the tissues were surface-sterilized with NaOCl for 5 min (Fig. 3B). This isolate was further confirmed as F. oxysporum f. sp. batatas SP-f6 by elongation factor 1-α gene sequence analysis. However, fungal mycelia were not isolated from the PDA-inoculated controls (Fig. 3B).
In vitro effect of gaseous chlorine dioxide against F. oxysporum f. sp. batatas SP-f6 on medium
When the in vitro inhibitory effect of gaseous ClO2 against F. oxysporum f. sp. batatas SP-f6 was tested on PDA, gaseous ClO2 at various concentrations and exposure times affected the growth of isolate SP-f6 (Fig. 4). Fungal population was significantly inhibited at 1 ppm of ClO2 for 30 min and was almost completely suppressed at 1 ppm for 60 min and 5 ppm for 5 min. Furthermore, ClO2 gas treatment at 5 ppm for 30–60 min and 10 and 20 ppm for 10–60 min completely inhibited the fungal population on medium (Fig. 4).
In vivo effect of gaseous chlorine dioxide against F. oxysporum f. sp. batatas SP-f6 on root slices
Lesion diameters caused by F. oxysporum f. sp. batatas SP-f6 in the inoculated sweetpotato slices decreased with an increase in ClO2 concentrations and exposure times in experiment 2, but not in experiment 1 (Fig. 5A, Supplementary Table 1). ClO2 concentration (P = 0.0013) and exposure duration (P < 0.0001) significantly affected lesion diameters on the inoculated slices in experiment 2, but not in experiment 1 (Supplementary Table 1). Significant concentration and time interactions (P = 0.9415 for experiment 1 and P = 0.1258 for experiment 2) for lesion diameters were not observed in either experiment (Supplementary Table 1). The degree of darkness of lesions declined over time, regardless of gas concentration. In addition, root slice tissues were lignified after gas treatment, especially at 20 ppm for 30–60 min (Supplementary Fig. 2). However, disease symptoms were not observed in uninoculated sweetpotato slices (Supplementary Fig. 2).
In vivo effect of gaseous chlorine dioxide against F. oxysporum f. sp. batatas SP-f6 on sweetpotato roots
When the in vivo inhibitory effect of gaseous ClO2 against F. oxysporum f. sp. batatas SP-f6 was tested on sweetpotato roots, growth of isolate SP-f6 was significantly inhibited by ClO2 gas treatment at 40 ppm for 30–60 min in experiment 1 and at 20 and 40 ppm for 60 min in experiment 2 (Fig. 5B). ClO2 gas treatment at 40 ppm inhibited more population of isolate SP-f6 than the gas treatment at 20 ppm for 30 and 60 min in experiment 1 (Fig. 5B). The concentration of ClO2 showed significant (P = 0.0001) inhibition on fungal population in an experiment; treated time exhibited significant (P = 0.0001 for experiment 1 and P = 0.0003 for experiment 2) reduction of fungal populations in both experiments (Supplementary Table 1). Significant (P = 0.0002) concentration and time interactions for fungal populations were observed in experiment 1 (Supplementary Table 1).
Discussion
In this study, we isolated fungal isolate SP-f6 from the black rot lesion on a diseased sweetpotato root and identified it as F. oxysporum f. sp. batatas using phylogenetic analysis of the elongation factor 1-α gene. Furthermore, we tested whether ClO2 gas had inhibitory activity against the development and growth of F. oxysporum f. sp. batatas on sweetpotato. We found that ClO2 gas suppressed fungal population and infection on sweetpotato roots. In addition, we observed that treatment time had a greater inhibitory effect than the ClO2 gas concentration on disease development and fungal population on sweetpotato.
ClO2 gas as a disinfectant has been widely used to control food-borne or postharvest microbial contamination on storage crops (Bhagat et al., 2011; Du et al., 2002; Yuk et al., 2006); thus, we examined the efficiency of ClO2 gas in controlling F. oxysporum f. sp. batatas–mediated infection in stored sweetpotato. In in vitro medium tests, the growth of F. oxysporum f. sp. batatas SP-f6 was significantly inhibited when treated with 1 ppm ClO2 gas for 30–60 min. Furthermore, growth of the fungus was completely inhibited following treatment with increased ClO2 concentration of 5 ppm for 30–60 min and 10 and 20 ppm for 10–60 min. In our previous study, Diaporthe batatas was shown to have similar growth inhibition pattern against various ClO2 concentrations and exposure times (Lee et al., 2019). There are several other studies that demonstrate in vitro inhibitory activity of ClO2 on various fungal contaminations on crops. Chen and Zhu (2011) reported that treatment of chestnut with 7 mg/l of ClO2 for 15 min led to a 4.6-log reduction of Fusarium tricinctum spores, whereas a 5.0-log reduction in Dothiorella gregaria spores was detected under the same treatment conditions. Wu et al. (2011) observed that spore germination rate of litchi anthracnose pathogens (Colletotrichum spp.) was significantly inhibited following treatment with 5 mg/l of ClO2 solution. In addition, Sun et al. (2017) found that aqueous ClO2 significantly inactivated spores of Fusarium graminearum, the causal agent of Fusarium head blight of wheat.
In in vivo inoculation tests on sweetpotato slices, lesion diameters were significantly restricted when the slices were treated with ClO2 gas for 30 min at 20 ppm treatment. Treatment for 60 min led to decrease in lesion diameters at all concentrations of the gas tested. However, lesion darkness, which indicates fungal growth and development, on slices turned lighter over time, and the tissues were lignified after gas treatment especially following the 60 mintreatment, regardless of the ClO2 concentration used. Tissue lignification resulting from gas treatment may not be a cause for concern as ClO2 gas is usually applied on sweetpotato roots. In in vivo root dip-inoculation tests followed by ClO2 gas treatment, fungal populations were reduced as observed in the slice tests. The inhibitory effect of ClO2 gas in this study followed a pattern similar to that observed in our previous study (Lee et al., 2019). These previous and present results imply that the ClO2 gas may have wide antifungal or antimicrobial activities beneficial for stored crops. In this regard, Bhagat et al. (2011) showed that ClO2 gas treatment inhibited Salmonella growth on oranges. They spot-inoculated a mixed culture of four Salmonella strains on the surface of orange and then treated the fruits with 0.1, 0.3, and 0.5 mg/l ClO2 gas for 2–14 min at 22°C and 90–95% relative humidity. Consequently, a 5-log reduction of Salmonella per sample was observed with 0.1 and 0.3 mg/l ClO2 gas treatments at 14 min, and 0.5 mg/l ClO2 gas at 10 min. Moreover, Sy et al. (2005) also found similar reductions in populations of foodborne pathogens such as Escherichia coli, Salmonella, Listeria monocytogenes, yeasts, and molds when the inoculated fresh and fresh-cut produces were exposed to 4.1 mg/l ClO2 gas.
Taken together, the results in this study showed that ClO2 gas can effectively inhibit fungal population and infection by F. oxysporum f. sp. batatas on sweetpotato. The ClO2 gas treatment with 40 ppm for 30–60 min may produce a desirable outcome by controlling the fungal growth and symptom development on the crop. Therefore, ClO2 gas may be used as a sanitizer to control F. oxysporum f. sp. batatas during postharvest storage of sweetpotato.
Supplemental Materials
Acknowledgments
This work was carried out with the supports of “Cooperative Research Program for Agriculture Science & Technology Development (Project No. PJ011332)” of Rural Development Administration, Republic of Korea and Korea University, Seoul, Republic of Korea. J.-J. Jeong was supported by the Global Ph.D. program through the National Research Foundation of Korea funded by the Ministry of Education (2015-034526) in Korea.
Notes
Electronic Supplementary Material
Supplementary materials are available at The Plant Pathology Journal website (http://www.ppjonline.org/).