Temperature, climate, salinity, nutrients and predators are important factors controlling the abundance and population dynamics of phytoplankton, including diatoms. In addition, viral infection poses a potential source of mortality, and several studies of algal-infecting viruses have appeared since the discovery of the first diatom virus, a single-stranded (ss) RNA virus that infects Rhizosolenia setigera (RsRNAV; Nagasaki et al., 2004).

Seawater contains many diverse organisms, including viruses and their hosts. One milliliter of seawater is estimated to contain 10⁵ to 10⁹ viral particles. Most of these viruses infect bacteria, cyanobacteria, archaea, and other eukaryotic organisms, but some infect macro- or microalgae. These algal viruses play an ecologically significant role in regulating the population dynamics of their phytoplankton hosts (Bratbak and Heldal, 1993; Suttle et al., 1990; Tarutani et al., 2000).

Over 50 viruses or virus-like particles (VLPs) infecting marine eukaryotic algae have been isolated and characterized over the past two decades (Nagasaki 2008; Nagasaki and Bratbak, 2010; Willson et al., 2009). To date, many algal host-virus systems have been established in laboratories and studied to various extents (Nagasaki, 2008; Van Etten et al., 1991; Van Etten and Meints, 1999). Among these species (excluding RsRNAV), at least nine diatom viruses have been isolated and characterized to date including Chaetoceros salsugineum nuclear inclusion virus (CsalDNAV, previously reported as CsNIV; Nagasaki et al., 2005), Chaetoceros nuclear inclusion virus (CspNIV; Bettarel et al., 2005) infecting Chaetoceros cf. gracilis, Chaetoceros debilis DNA virus (CdebDNAV; Tomaru et al., 2008), Chaetoceros tenuissimus RNA virus (CtenRNAV; Shirai et al., 2008), Chaetoceros socialis f. radians RNA virus (CsfrRNAV; Tomaru et al., 2009), Chaetoceros cf. wighamii nuclear inclusion virus (CwNIV; Eissler et al., 2009), Chaetoceros lorenzianus DNA virus (ClorDNAV; Tomaru et al., 2011); Chaetoceros sp. DNA virus (Csp05DNAV; Toyoda et al., 2012), and Chaetoceros setoensis DNA virus (CsetDNAV; Tomaru et al., 2013).

The present study reports the isolation and physiological characterization of a new diatom virus, ScosV, which infects S. costatum from Korean coastal water.

Seawater samples were collected from Jaran Bay, Korea, between May and October 2008. In the laboratory, samples were filtered through 0.8 μm pore-size polycarbonate filters (Nuclepore, VA). The host organism, S. costatum (strain ME-SCM-1), was provided by the National Fisheries Research and Development Institute in Korea and was cultured in modified f/2 medium (Gillard, 1973) under a 12L:12D cycle of 80 to 90 μmol photons/m²/s with cool white fluorescent illumination at 20°C. Logarithmic-phase cultures of S. costatum (500 ml) were inoculated with 20 ml of the filtered sea water and incubated under the same conditions as above. Cultures and cells of S. costatum were lysed by the filtrate and became pale in color, presumably due to the loss or degradation of photosynthetic pigments. To confirm that virus particles were present, a lysate of a S. costatum culture was filtered through 0.2 μm pore-size Dismic-25cs filters (Advantec, Charlotte, NC). Incubation with the viral lysate caused complete lysis of host cultures within 1 week, whereas controls remained healthy (Fig. 1A, B). Further cloning of the virus strain was performed following the method of Tarutani et al. (2001), and the isolated virus was named S. costatum infecting virus (ScosV).

The host range of ScosV was examined by adding 50 μl of the original pathogen suspension to 1 ml of exponentially growing cultures of the algal strains listed in Table 1. Each culture was incubated under the appropriate conditions and observed via optical microscopy. ScosV was not lytic to any of the microalgal species tested, other than S. costatum; moreover, it was not lytic to any other diatom strain tested (Table 1). The infectivity of ScosV was therefore considered to be not only species-specific, but also strain-specific, similar to several other algal viruses (Tomaru et al., 2004a, 2004b, 2008). The strain specificity of an algal virus is important for the maintenance of intraspecies diversity in algal populations in nature. The number of host strains tested in the present study was limited, but isolations of more virus isolates and host stains will elucidate the dynamic relationships between S. costatum and viruses infecting this host species.

The replication parameters of ScosV were determined using growth experiments. Cultures containing 500 ml of exponentially growing host cells were inoculated with 20 ml of lysate containing ScosV at a viral titer of 2.67×10⁷, estimated using the most probable number (MPN) method; the cell density and virus titer were then measured under light microscopy and using the extinction dilution method, respectively (Tarutani et al., 2001), every 12 h until reaching 80 hours post inoculation (hpi) (Fig. 2). An increase in viral titer was detected from 24 to 72 hpi; thus, the lytic cycle of ScosV was predicted to be <48 h. Furthermore, by comparing the decrease in host cell abundance to the increase in viral titer from 48 to 72 hpi, the burst size was estimated at 90-250 infectious units/cell. The latent period of ScosV was similar to those of other microalgal viruses (Nagasaki et al., 2003) and fell between those previously reported for diatom-infecting viruses such as CsNIV (Nagasaki et al., 2005) and CspNIV (Bettarel et al., 2005), which were 40-56 h and 24-48 h, respectively. However, the estimated burst size was markedly smaller than that of CsNIV, which has a burst size of 325 infectious units per host cell.

The morphology of ScosV was observed via transmission electron microscopy (TEM). S. costatum cultures were inoculated with ScosV, and 10-ml samples were collected at 0, 12, 24, 36, 48, 60, 72, 84, and 96 hpi and fixed with 1% glutaraldehyde in f/2 medium for 2 h at 4°C. Cells were harvested by centrifugation at 3,000 rpm for 20 min and then post-fixed for an additional 1 h with 2% osmium tetroxide at 4°C. Following two rinses with phosphate-buffered saline (PBS), the pellet was dehydrated in a graded ethanol series (20-100%) and embedded in Poly/Bed^® 812 resin (Polyscience, Inc., Warrington, PA). Thin sections were stained with 4% uranyl acetate and 3% lead citrate and observed under a TEM (JEOL JEM-1010; JEOL Ltd., MA) with an acceleration voltage of 60 kV. VLPs that were negatively stained with 4% (w/v) uranyl acetate were also observed under TEM. Briefly, a drop of purified virus suspension was mounted on a grid for 30 s, and excess water removed using filter paper. After the grid had dried, negatively stained VLPs were observed via TEM under the same conditions. Particle diameters were estimated on negatively stained images.

Intracellular VLPs were observed 72 h after inoculation with ScosV. These VLPS were ~38-46 nm in diameter and appeared as electron-opaque particles in the cytoplasm (Fig. 3B, C and D). No typical crystalline array formation was observed. VLPs were not found in healthy control cells (Fig. 3A). Virus particles appeared hexagonal in outline upon negative staining, were 40±10 nm in diameter had no outer membrane or tail-like structure (Fig. 3E), and were similar to VLPs observed in the cytoplasm (Fig. 3D). The size, icosahedral morphology, and cytoplasmic site of virus assembly are properties shared by algal virus VLPs (Van Etten et al., 1991), including C. salsugineum VLPs (38 nm; Nagasaki et al., 2005) and C. lorenzianus VLPs (34±2 nm; Tomaru et al., 2011). Because 1) the algicidal pathogen was transferable to a fresh algal culture, 2) VLPs were observed in the culture lysate, and, 3) VLPs were not observed in healthy cultures, we concluded that the VLPs observed in cytoplasm were pathogenic to S. costatum.

The effect of storage temperature on ScosV infectivity was examined following the methods of Tomaru et al., (2005). An exponentially growing culture of S. costatum was inoculated with the virus and incubated for 4 days. The resultant lysate was sequentially passed through 0.8 μm and 0.22 μm filters to remove cell debris. The titer of the fresh lysate, estimated using the extinction dilution method, was 2.7×10⁷ infectious units/ml. Aliquots of the lysate were stored at 20, 15, 4, and −196°C (liquid nitrogen) under light or dark conditions with or without 10% or 20% dimethyl sulfoxide (DMSO) as a cryoprotectant, and were then re-titrated using the extinction dilution method. After 2 weeks of storage at 4, 15, and 20°C in the dark, the estimated titers were 5.1×10⁵, 7.74×10⁴, and 2.3×10³ infectious units/ml, respectively; however, titers fell below the detection limit (<3.0×10¹ infectious units/ml) after 4 months of storage at each temperature. Decreases in infectious titers have been reported for other microalgal viruses, such as CtenRNAV and CsfrRNAV, which exhibit considerable decay even under cold and dark conditions (Shirai et al., 2008; Tomaru et al., 2009). Based on these results, a significant loss of infectivity may occur in natural environments. Cryopreservation conditions for ScosV were optimized using a method similar to those of Nagasaki and Yamaguchi (1999) and Tomaru et al. (2005); the highest titers (2.1×10⁵ infectious units/ml) after 1 month of storage were recorded when the viral suspension was preserved in liquid nitrogen (−196°C) with 10% DMSO as a cryoprotectant (initial concentration = 100%; Merck, NY). The temperature stability of the diatom virus group is high (Tomaru et al., 2008, 2011; Toyoda et al., 2012).

Viruses are thought to influence community composition by infecting principally competitively superior host species that are abundant and fast-growing (Sandaa, 2008; Wommack and Colwell, 2000). Although we do not have sufficient data to determine the roles played by viruses in marine environments, or in host population dynamics, the interaction between Phaeocystis pouchetii and the lytic virus PpV01 in laboratory microcosms (Bratbak and Heldal, 2000) and the ecological relationship between the bloom-forming dinoflagellate Heterocapsa circularisquama and its infectious virus, HcRNAV, afford plausible explanations of the roles played by viruses in the dynamics of host algae (Nagasaki et al., 2003; Tomaru and Nagasaki, 2004).