Characterization of a Korean Isolate of Dasheen mosaic virus Isolated from Taro(Colocasia esculenta Schott) in Korea

Kim, Min Kyu; Kwon, Soon Bae; Yoon, Ju Yeon; Ryu, Ki Hyun; Heo, Su Jeong; Hong, Jeong Ki; Kim, Kyung Hee; Choi, Jang Kyung

The Plant Pathology Journal 2004;20(2):135-141.
Published online June 30, 2004.

Characterization of a Korean Isolate of Dasheen mosaic virus Isolated from Taro(Colocasia esculenta Schott) in Korea

Min Kyu Kim, Soon Bae Kwon, Ju Yeon Yoon, Ki Hyun Ryu, Su Jeong Heo, Jeong Ki Hong, Kyung Hee Kim, Jang Kyung Choi

Abstract

A filamentous virus was isolated from taro (Colocasia esculenta Schott) showing mosaic and chlorotic feathering symptoms in Chuncheon, Gangwon province in 2002. Based on ELISA, its appearance in electron microscope, serological relationships, and RT-PCR using specific primer and nucleotide sequence analysis of the CP gene, the isolated virus was identified as Dasheen mosaic virus (DsMV) and designated as Korean isolated (DsMV-Kr). DsMV was not serologically related to Zantedeschia mosaic virus (ZaMV), which has been reported to infect an Araceae plants. Since the coat protein revealed electrophoretic heterogeneity, about 42 kDa, 39 kDa and 31 kDa by SDS-PAGE, an improved purification method was established for the production of antisera against DsMV-Kr. The purification method used in this study may be effectively applied to the purification of other filamentous viruses.

Key Words: Colocasia esculenta Schott, Dasheen mosaic virus, Zantedeschia mosaic virus